The synthesis and characterization of a triazole linked quinoline appended calix[4]arene conjugate, L, and its fluorescence turn on receptor property for Fe3+ have been demonstrated. The selective and sensitive discrimination of Fe3+ has been shown using fluorescence and absorption titration experiments. The Fe3+ binding to L has been further shown by ITC and ESI MS. The mode of binding of Fe3+ by calix[4]arene conjugate has been shown by absorption, 1H NMR and visual color change and the species were modeled based on DFT computations. The {L + Fe3+} has been shown to label cells with fluorescence imaging. Moreover the utility of this conjugate has been demonstrated by the combination logic gate system. © 2013 American Chemical Society.