RuelliapatulaJacq (syn: Dipteracanthus patulus) belongs to the family Acanthaceae, has numerous medicinal properties but is not exploited much in modern medicine. Invitro antimicrobial activity of the Ruelliapatulajacq leaves extracted with Ethanol, Methanol and Acetone was checked for Bacillus subtilis, Escherichia coli and Aspergillus niger by disc diffusion method. Ethanol was found to be the better solvent that its extract showed more activity against Aspergillus niger, Escherichia coli and Bacillus subtilis respectively. Preliminary phytochemical screening of ethanolic and methanolic extracts showed positive results for alkaloids, steroids, phenols, flavonoids, tannins and terpenoids. Genomic DNA was extracted from RuelliapatulaJacq leaves using the standard Cetyl Trimethyl Ammonium Bromide extraction method. The DNA extracted responded well during PCR amplification and RAPD analysis with three gene specific primers and five random Medicinal aromatic plant primers respectively. Chromatographic fingerprinting of the ethanolic extract of the plant leaves was analyzed for compounds present in the sample using analytical type HPLC using a C-13 Column and a UV- detector of the RuelliapatulaJacq for the detection of the chemical constituents in the plant. Analytical type chromatogram revealed the presence of 8 compounds in the extract under scanning at 205 nm. The preparative type HPLC chromatogram revealed the presence of 2 major compounds in the extract. The collected fractions were subjected to FT-IR spectroscopy and UV-Visible spectrum analysis. FT-IR Spectrum studies on RuelliapatulaJacq ethanolic extract showed the possibility of harbouring secondary metabolites with higher pharmaceutical value.