Biological carbon sequestration gaining more impetus day by day, where algae is the main backbone as a biological agent. Thus, the present study focused on the evaluation of algal biomass growth, collected from the polysheet wall of green house chamber, along with its carbon capture potentiality. The experiment were conducted in three different atmospheric conditions viz. (i) inside the green house chamber where temperature and carbon dioxide concentration was higher; (ii) in the normal atmospheric condition under full sunlight, and (iii) in closed room condition where light intensity was fixed. Maximum carbon accumulation was found (834.59±2.09 mg C/g) inside the green house chamber where mean temperature and carbon dioxide concentration were 41 °C and 705 ppm respectively. Collective effect of temperature and carbon dioxide concentration also facilitate in the maximum growth of the algal biomass (8693.68 mean number of cell/mL) in the greenhouse chamber.