The effects of 4.0, 8.0, 10.0, and 20.0 mM aqueous sodium fluoride (NaF) were studied on Bengal gram seedlings, Cicer arietinum L. A significant decrease (p<0.01) in the physiological growth parameters (root and shoot length, fresh root and shoot weight, dry root and shoot weight, number of primary leaves, and number of root branches) was observed with increasing F concentration after seven days of treatment. However, separate addition of aqueous calcium chloride (CaCl2) to the NaF growth media gave little or only minor improvement in the growth physiology of the seedlings. On the other hand, the pigment, protein, and proline content showed good agreement with F stress, and addition of CaCl2 caused improvement in all biochemical parameters. The concentration of F in the plant body increased with increasing NaF concentration, but maximum reduction of plant body F occurred with the addition of 10.0 mM CaCl2 to the 20mM NaF growth medium. © 2012 The International Society for Fluoride Research Inc.