Bacterial infections and the rise of antibiotic-resistant strains pose a critical challenge to public health and has intensified the need for alternative antimicrobial agents. This study explores the synthesis, characterization, and antibacterial efficacy of hexagonal Bi2Te3 platelets prepared via a solvothermal method using polyvinylpyrrolidone (PVP) as a stabilizer, ensuring high dispersion stability and preventing agglomeration. The synthesized Bi2Te3 nanoparticles were thoroughly characterized using X-ray diffraction (XRD), scanning and transmission electron microscopy (SEM, TEM), Raman and Fourier-transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS), confirming the crystalline quality, surface properties, and functional groups of the Bi2Te3 platelets. The antibacterial efficacy was assessed using well-diffusion assays and minimum inhibitory concentration (MIC) tests, demonstrating effective bactericidal action, particularly against Gram-positive bacteria, attributed to membrane disruption and reactive oxygen species (ROS) generation. Additionally, cytotoxicity studies on A549 lung carcinoma cells indicated dose-dependent effects, supporting the biocompatibility of Bi2Te3 at optimal concentrations. The scalable of hexagonal Bi2Te3 platelets position them as promising candidates for advanced antibacterial applications.