The feasibility of power generation by non-destructive usage of rhizodeposits of Pennisetum setaceum plant formed mainly due to photosynthesis-carbon sequestration mechanism was studied in rhizosphere based microbial fuel-cell (R-MFC). Four fuel-cell assemblies (non-catalyzed graphite-plates; membrane-less operation; air-cathode) were evaluated for their electrogenic activity by varying anode distances from root in rhizosphere [A1 – 0; A2 – 8; A3 – 12 and A4 – 16. cm] at 2. cm depth from soil-layer and analyzed their electrogenic potential. The fuel-cell assembly near to the root zone showed maximum electrogenic-activity (R1, 1007. mV/4.52. mA) followed by R2 (780. mV/4.11. mA), R3 (720. mV/3.4. mA) and R4 (220. mV/1.2. mA). The observed maximum electrogenesis with R1 and minimum with R4 electrode-assemblies enumerated the critical role of root-exudates as substrates. All fuel-cell assemblies showed 10% higher electrogenic activity during day-time operation which can be directly attributed to plant’s photosynthetic activity. The study enumerated the potential of plant to harness power in a sustainable way by optimum placement of fuel-cell setup in their rhizosphere. © 2012 Elsevier Ltd.