Medical diagnosis has been developed with new techniques which are capable of performing very sensitive detection and quantifying certain parameters. Microfluidic based sensors are taking very essential part in the diagnosis of several parameters. These parameters can be correlated with the presence of specific molecules and their quantities. A lab-on-chip biosensor is a miniaturized device integrated in a single chip which can perform one or several analyses including human diagnostics done in the laboratory. This work presents, design and model of a lab on chip biosensor with molecule parameters using COMSOL multi-physics. In this paper, designed a glucose sensor, which can be used to track the glucose levels in body which helps diabetic patients maintain their glucose levels. The aim of this work is to design of a glucose sensor which is highly sensitive. The sensor is designed with an electrode and reaction surface in a micro channel. The designed sensor harvests a decent sensitivity in terms of average current density and with a limit-of-detection value 0.01µM.