Subgrade soil is a critical component of highway construction. In general, a subgrade soil can be made using locally available materials. The performance parameters of soils must be modified to ensure the long-term sustainability of structures built on them. In the field of geotechnical engineering, the bio-enzymes can be used for the stabilization of weak soil produces positive results. Most countries have recently begun to produce and utilize bio-enzymes as additives to improve soil stability and usefulness in soil stabilization. Bio-enzymes can lower the cost of construction while also providing the best output from the stabilization process. It is simple to make bio-enzymes utilizing local resources with a little research and experience. Bio-enzymes can be used in ground improvement projects such as pavements and embankments by performing some laboratory tests and field experiments. The efficiency of soil stabilizer and the need for adequate soil grading as per project requirements are explained by the combination of laboratory tests and field execution. The purpose of this research is to find out what engineering properties soil has after treating the soil with bio-enzyme and investigate the effect of changing the bio-enzyme dosage on the different properties of soil, and several strength parameters like California Bearing Ratio and unconfined compressive strength (UCS) were calculated using standard sized samples at curing periods of 0, 7, 14, 21, 28 days. Then to discover the properties of modified (stabilized) soil. Here, we will obtain the optimum measure of bio-enzyme at which soil will acquire maximum strength.