Bacterial cellulose is a biopolymer whose interest in the global market is increasing because of its advanced properties concerning the high purity, fibril structure, high degree of polymerization, and high crystallinity. To enhance the production of bacterial cellulose at pilot plant scale, new approaches are tried upon with respect to culture condition, agitation in bioreactors, supplementation of nutrition, and its cost effectiveness. This chapter also discusses on the genetically modified strain for enhanced cellulose production. In addition, bacterial cellulose has immense potential in many industrial sectors such as paper industries, textile industries, electronics, food industries, and biomedical devices. It plays a major role in tissue engineering with application in wound care and revival of the damaged organs. Moreover, it is having multifunctional properties in food industries and is engaged in maintaining shelf life, as thickening and gelling agent, and as a packaging material due to its water binding capacity. Bacterial cellulose shows good barrier properties in preventing food rancidity and also improves the food rheology. Due to the unique properties of bacterial cellulose, it is attractive innovative products have been developed and commercialized making it an attractive biopolymer for industrial application.