This chapter deals with various biomedical applications of MXenes, from biosensing, photothermal and photodynamic anticancer therapy, combination anticancer therapy with other treatment modalities, theranostics, antimicrobial treatment platforms to more recent tissue engineering applications. The chapter throws light on the mechanistic understanding of the role of MXenes in various types of biosensors fabrication, with an emphasis on wearable sensors. The chapter also gives a detailed understanding of MXenes’ role as photothermal, photodynamic, and theranostic agents. The chapter also explicates the importance of MXenes in antimicrobial treatment and their relevance in treating antimicrobial resistance. Finally, the chapter also discusses the biocompatibility studies on MXene, which are an important aspect for MXene to have continued usage in biomedical applications. Overall, this chapter gives an overview of various biomedical applications and the relevance of MXenes in each of these applications.