Plants produce a vast array of metabolites, far more than those produced by most other organisms. Large-scale metabolite profiling assays now provide unmatched access to global data sets of metabolites and their correspond ing pathways, greatly enhancing our understanding of plant biology. High-throughput metabolome analysis platforms have accelerated the discovery of diverse biochemical metabolites and new pathways while enhancing our understanding of existing ones. Despite many metabolites remaining unidentified, metabolomics has signifi cantly advanced our comprehension of plant physiology and biology through the study of these small molecules. As well as the latest advancements in analytical techniques, the integration of metabolomics with other omics will greatly enhance our understanding of biological systems. In this chapter, we briefly describe the latest developments in the field of analytical techniques and their application in plant metabolomics.