Abstract
Three-dimensional (3D) bioprinting technology enables the fabrication of porous structures with complicated and variable geometries, allowing for the equitable distribution of cells and the regulated release of signalling components, which distinguishes it from traditional tissue scaffolding approaches. In 3D bioprinting, various cell-laden materials, including organic and synthetic polymers, have been used to create scaffolding systems and extracellular matrix (ECM) for tissue engineering (TE). However, significant technological hurdles remain, including bio-ink composition, printability, customizing mechanical and biological characteristics in hydrogel implants, and cell behaviour guiding in biomaterials. This chapter investigates several methodologies for hydrogel-based bio-inks that can mimic the ECM environment of real bone tissue. The study also looks at the process factors of bio-ink formulations and printing, as well as the structural requirements and production methods of long-lasting hydrogel scaffolds. Finally, contemporary bioprinting techniques are discussed, and the chapter concludes with an overview of the existing obstacles and probable future prospects for smart hydrogel-based bio-inks/scaffolds in tissue regeneration.
