Remediation of pesticides by advanced oxidation process gains enormous interest due to its feasible applications at the polluted site. The 2,4-dichlorophenoxyacetic acid (2,4-D), a common herbicide in water bodies, poses a major environmental threat to humans and aquatic organisms. However, the advanced oxidation process offers a possible solution for its effective recovery. Optimisation of the critical parameter will support the possible recovery process of the 2,4-dichlo-rophenoxyacetic degradation. In the present study, response surface methodology based analysis of variance optimization was made for a modified TiO2 catalyst in a glass fabricated photo-catalytic reactor for 2,4-dichlorophenoxyacetic acid degradation. The variables investigated were pH (2–10), initial 2,4-D concentration (10–100 mg/L), and catalyst loading (25–150 mg/L). The maximum removal efficiency of 97% has been achieved at the optimized variable of 87.5 mg/L of catalyst dosage at 55 mg/L of 2,4-D concentration at pH 6.